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产品名称: Phi29 DNA Polymerase
产品型号: E014
产品展商: 其他品牌
产品文档: 无相关文档
简单介绍
Phi29 DNA Polymerase
Phi29 DNA Polymerase
的详细介绍
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Print Version |
| Description |
Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates. |
| Application |
• Rolling circle amplification (RCA)
• Multiple displacement amplification (MDA)
• Whole genome amplification (WGA)
• DNA template preparation for sequencing
• Protein-primed DNA amplification |
| Form |
Enzyme supplied with 5X Reaction Buffer |
| Image |
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| Concentration |
10 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, 0.5% Tween-20, 0.5% NP-40 and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of Phi29 DNA Polymerase that is required to incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use . |
