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产品名称: E. coli DNA Polymerase I
产品型号: G469
产品展商: 其他品牌
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简单介绍
E. coli DNA Polymerase I
E. coli DNA Polymerase I
的详细介绍
|
Print Version |
| Description |
E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity. |
| Application |
Generation of labeled DNA probes by nick translation
Second-strand synthesis of cDNA
Note: This product is not suitable for NGS or second strand cDNA synthesis applications. |
| Form |
Enzyme supplied with 10X Reaction Buffer |
| Image |
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| Concentration |
5 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use. |
