DNA Polymerase I Large (Klenow) Fragment
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产品名称: DNA Polymerase I Large (Klenow) Fragment
产品型号: E013
产品展商: 其他品牌
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简单介绍
DNA Polymerase I Large (Klenow) Fragment
DNA Polymerase I Large (Klenow) Fragment
的详细介绍
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Print Version |
| Description |
DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini. |
| Application |
DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs
cDNA second-strand synthesis
Generate single-stranded DNA probes using random primers
Site-directed DNA mutagenesis using synthetic oligonucleotides
Dideoxy DNA sequencing of single- or double-stranded DNA templates
3’→5’ exonuclease activity can blunt a 3’-overhang |
| Form |
Enzyme supplied with 10X Reaction Buffer |
| Image |
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| Concentration |
5 U/μl |
| Storage |
Store all components at -20°C. |
| Storage Buffer |
25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. |
| Notes |
One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use. |
