qPCR Adeno-Associated Virus Titration (Titer) Kit

Description

  The qPCR Adeno-Associated Virus Titration (Titer) Kit provides a fast and simple way to perform Adeno-Associated Virus titration. This kit employs a quick DNA extraction step and determines viral DNA titer using a qPCR assay that can be completed within 2 hours. abm’s kit has demonstrated itself to be superior in both sensitivity and specificity compared to similar products on the market, while keeping non-specific background signals to a minimum.
  Viral DNA is isolated from a small aliquot of unknown samples using the Virus Lysis Buffer provided. The isolated DNA is then included in the qPCR assay with two known AAV standards to determine threshold cycle (Ct) values. A final viral titer of the unknown samples can then be determined by an AAV-titer calculation using resulting Ct values.

Advantages of ABM's AAV qPCR Titer Kit

• no DNA purification step required, saves time and eliminates inaccuracies
• ready to use reagent mix reduces variability
• rapid set-up, qPCR assay can be run in under 2 hours
• quantitative assay

Titer Calculator

Average Ct of STD1       Average Ct of STD2    
Average Ct of Sample    

                  Titer of sample:

GC/ml

Note: STD1, STD2 are provided in the AAV qPCR Titer Kit.