qPCR Lentivirus Complete Titration Kit (MasterMix-LR)

Description

  The qPCR Lentivirus Complete Titration Kit provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. This complete kit differs from the standard qPCR Lentivirus Titration Kit (Cat. No. LV900) in that it contains the Lentivirus 2X qPCR MasterMix necessary for the real-time PCR reaction. Please refer to our qPCR MasterMix Selection Guide for selecting the appropriate qPCR formulation applicable to your particular instrument model. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.

User Manual  

Advantages of ABM's Lentiviral qPCR Titer Kit

• no RNA purification -- saves time and eliminates inaccuracy
• ready-to-use reagent mix -- reduces variability
• qRT-PCR in one step -- more sensitive and accurate than other methods
• no NTC amplification -- our titration kit is the only one on the market that completely eliminates NTC signal due to our unique primer design. Similar kits from other companies all give NTC amplification, which compromise accuracy for viral titration

Shippping Conditions

Ships on dry ice. Upon arrival, the qPCR Lentivirus Titration Kit should be stored at -20°C. Avoid repeated freeze-thaw cycles to retain maximal performance. The kit is stable for 1 year from the ship date when stored and handled properly.

Titer Calculator

Titer Calculator

Average Ct of STD1       Average Ct of STD2    
Average Ct of Sample    

                  Titer of sample:

Note: STD1, STD2 are provided in the Lentivirus qPCR Titer Kit.