|
Print Version |
| BioSafety Level |
II |
| Organism |
Large White Swine |
| Source Organ |
Aorta |
| Growth Properties |
Adherent |
| Morphology |
Cobblestone-like |
| Recommended Seeding Density |
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers |
CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 |
| Applications |
For Research Use Only |
| Immortalization Method |
Tranfection with pRNS-1 plasmid encoding the SV40 genome |
| Cell Type Characterization |
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| Description |
Recently proposed as a potential model of atherosclerosis, allograft rejection and accommodation, xenotransplantation and viral haemorrhage diseases, the porcine endothelial cells provide a useful tool in studies including the molecular mechanisms of xenogeneic rejection in the swine-to-human combination, and the pathogenesis of African Swine Fever (ASF) and Classical Swine Fever (CSF). The Immortalized Porcine Aortic Endothelial Cells (AOC) is derived from stable transformation of SV40 genome into primary porcine aortic endothelial cells. The resulting AOC retains comparable surface markers to its primary cell counterparts, as well as preserving its ability to uptake acetylated low density lipoproteins (Ac-LDL) and its susceptibility to xenogeneic cell-mediated cytotoxicity (i.e. Human Natural Killer Cell-mediated lysis). |
| Procedure Overview |
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| Propagation |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow II medium available from ABM (TM002). To make the completed growth medium, add the following components to the base medium: 20% fetal-bovine serum (TM999) and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Preservation |
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase. |
| Quality Control |
1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD29, CD31, CD41/61, CD80/86, CD46, SWC3, LAMP-1 antigen and MHC Class I antigens; 2) 51Cr release assay was used to analyse the susceptibility of AOC to xenogeneic cell-mediated cytotoxicity; 3) 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine (DiI)-labelled Ac-LDL was used to evaluate Ac-LDL update by the AOC. |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
