Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)
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产品名称: Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)
产品型号: T0129
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简单介绍
Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)
Immortalized Mouse Embryonic Hepatic Progenitor Cells (iHP)
的详细介绍
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Print Version |
| BioSafety Level |
II |
| Organism |
CD1 Mouse Embryo |
| Source Organ |
Liver |
| Growth Properties |
Adherent |
| Morphology |
Polygonal |
| Recommended Seeding Density |
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Markers |
CD34, Pou4f1/Oct4, AFP, Dlk, Alb |
| Applications |
For Research Use Only |
| Immortalization Method |
Serial passaging and transduction with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites |
| Description |
The Immortalized Mouse Embryonic Hepatic Progenitor Cells are non-tumorigenic, hygromycin-resistant, reversibly immortalized cells that expresses endothelial (CD34), progenitor (Pou5f1/Oct4), and hepatic (AFP, Dlk, Alb) cell markers. The cells are programmed for liver-specific gene expression and differentiation, as indicated by the presence of hepatic/pancreatic transcriptions factors (i.e. HNF3/Foxa1, HNF3β/Foxa2, and HNF4α/MODY1) that are involved in mouse liver development. Indocyanine green (ICG) uptake and glycogen storage functions has also been observed in these cells.
The cells are unique as the immortalization process may be reversed by removing SV40 large T antigen expression via transduction with recombinant adenovirus expressing Cre recombinase (abm Cat. No. 000018A), resulting in primary hepatic progenitor cells. Both immortalized and primary forms of the hepatic progenitor may undergo differentiation in vitro when cultured with dexamethasone, developing mature hepatocytes which express hepatic cell markers (i.e. CK18, TAT, and ApoB). It is recommended to use these cells for studies in liver stem cell, liver organogenesis, and hepatocytes transplant therapy.
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| Procedure Overview |
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| Propagation |
Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
To differentiate cells: Add dexamethasone to a final concentration of 1 μM to the complete growth medium.
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| Quality Control |
1) qPCR to examine expression of cell markers; 2) Western blot analysis for SV40 large T antigen; 3) Indocyanine green (ICG) uptake and release assay and periodic acid-schiff (PAS) staining were performed to determine cell functions. |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
