TrkA Stable T17 Cell Line
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产品名称: TrkA Stable T17 Cell Line
产品型号: T3158
产品展商: 其他品牌
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简单介绍
TrkA Stable T17 Cell Line
TrkA Stable T17 Cell Line
的详细介绍
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Print Version |
| BioSafety Level |
II |
| Organism |
Mouse |
| SourceOrgan |
Brain |
| Growth Properties |
Adherent |
| Morphology |
Polygonal |
| Description |
TrkA Stable T17 Cell Line expresses rat TrkA (Tropomyosin receptor kinase A) after TrkA plasmid was successfully transfected into SN56 cells and were selected in the presence of G418. TrkA is the receptor for neurotrophins, such as nerve growth factor (NGF), which plays essential role in the nervous system – in particular apoptotic cell death prevention, and/or the promotion of cellular differentiation and axon elongation in neurons. Researchers may use the TrkA Stable T17 Cell Line to screen for NGF-like compounds that would act as TrkA agonists to study the mechanisms of the signaling pathways and affects NGF. The lack of TrkA has been associated with the neurodegenerative processes and thus the cell line is a valuable tool to conduct related studies.
abm has available the TrkB Stable T48 Cell Line (T3159), which can be used to screen BDNF-like (brain-derived neurotrophic factor –like) compounds as well.
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| Procedure Overview |
 |
| Recommended Seeding Density |
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Applications |
For Research Use Only |
| Over Expression |
Rat TrkA receptor via transfection and G418 antibiotic selection |
| Propagation |
Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to final concentration of 10% fetal bovine serum (TM999), 1 mM Sodium Pyruvate Solution(TM057), 1% Penicillin/Streptomycin (G255), and 300 mg/ml G418 (G271). Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. |
| Pictures |
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| Quality Control |
Selection with 300 mg/ml G418 |
| References |
Jang, S., Okada, M., Sayeed, I., Xiao, G., Stein, D., Jin, P., & Ye, K. (2007). Gambogic amide, a selective agonist for TrkA receptor that possesses robust neurotrophic activity, prevents neuronal cell death. Proceedings of the National Academy of Sciences, 104(41), 16329-16334. doi:10.1073/pnas.0706662104 |
| Disclaimer |
1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
