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MEF-iPSC

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产品名称: MEF-iPSC
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MEF-iPSC


MEF-iPSC  的详细介绍
Print Version
Safety BioSafety Level II
Organism Mouse
Source Organ Embryo
Concentration 106cells/vial
Propagation The base medium for this cell line is Prigrow X series medium available in ABM, Cat. No. TM2019
Atmosphere:air: 95%, CO2: 5%; Temperature: 37.0°C.
Subculturing 1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new feeder cell culture vessel. 6. Incubate cultures at 37°C. Be sure to use feeder cells (MEF, T2009 or REF T2017).
Storage -180°C
Shipping On Dry Ice
Notes For Research Use Only, not for therapeutic or diagnostic purposes.
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