Catalogue number
CYT-402
Synonyms
Growth Differentiation Factor 11, GDF-11, Bone Morphogenetic Protein 11, BMP11.
Introduction
GDF-11 belongs to the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. GDF-11 is a central developmental factor which controls muscular and neural development. In *****s, GDF-11 encourages cardiac hypertrophy reverse by the revival of cardiomyocytes.
Description
GDF11 Human Recombinant produced in E.Coli is a non-glycosylated homodimer containing 2x109 amino acids and having a total molecular mass of 24.9kDa.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA).
Solubility
It is recommended to reconstitute the lyophilized GDF11 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized GDF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF11 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL
VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS
Biological Activity
The ED50 as determined by the ability to inhibit alkaline phosphatase activity in ATDC5 cells, is 0.2-0.3ng/ml, corresponding to a specific activity of 5 x 106units/mg.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
