Interleukin-33, IL-33.
nterleukin 33 (IL-33) is a 32kDa proinflammatory cytokine that may also regulate gene transcription in producer cells. IL-33 is structurally related to IL-1, which induces helper T cells to produce type 2 cytokines and acts through the receptor IL1RL-1 (IL1 receptor-like-1), which is known also as ST2. Binding of IL-33 to this receptor activates NF-kappa-B and MAP kinases and induces in vitro Th2 cells to produce cytokines. In vivo, IL-33 induces expression of IL-4, IL-5, IL-13 and leads to severe pathological changes in mucosal organs and in vitro, it can be divided to N-terminal fragment of 12kDa and C-terminal fragment of 18kDa by cleavage of caspase-1.
IL 33 Rat Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 179 amino acids (109-264 a.a) and
having a molecular mass of 19.8kDa.
IL 33 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Escherichia Coli.
Sterile Filtered colorless solution.
IL 33 protein solution (0.5mg/ml) containing Phosphate Buffered
Saline (pH7.4) and 10% glycerol.
Store at 4°C if entire vial will be used within 2-4
weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Greater
than 90.0% as determined by SDS-PAGE.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
