Catalogue number
ANT-545
Synonyms
BUB3L, hBUB3, Mitotic checkpoint protein BUB3.
Type
Mouse Anti Human Monoclonal.
Introduction
Mitotic checkpoint protein BUB3 (BUB3), is a saved component of the mitotic spindle assembly complex (MCC). The encoded protein has 4 WD repeat domains and has a similar sequence as the yeast BUB3 protein. BUB3 is vital for the kinetochore localization of BUB1 and BUBR1. BUB3 participates in the central spindle checkpoint pathway that operates during early embryogenesis. Furthermore BUB3 has a part in regulating the establishment of correct kinetochore-microtubule attachments.
Clone
PAT2H6AT.
Physical Appearance
Sterile filtered colorless solution.
Immunogen
Anti-human BUB3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human BUB3 protein 1-328 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain.
Purification Method
BUB3 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Formulation
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.
Prevent freeze thaw cycles.
Stability / Shelf Life
12 months at -20°C. 1 month at 4°C.
Applications
BUB3 antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
