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SNRPD Human

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简单介绍

SNRPD Human


SNRPD Human  的详细介绍

Catalogue number

PRO-997

Description

SNRPD Human Recombinant produced in SF9 insect cells, is a mixed protein complex containing the SNRPD1 (14,306 Dalton), SNRPD2 (14,773 Dalton) and SNRPD3 (14,739 Dalton) proteins.
Each protein (SNRPD1+ SNRPD2+ SNRPD3) is individually fused to a -6xHis tag at N-terminus and purified by proprietary chromatographic techniques.

Source

Sf9 insect cells.

Physical Appearance

Sterile Filtered clear solution.

Formulation

SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Purity

Greater than 80% as determined by SDS-PAGE.

Coating concentration

0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Applications

Western blot with:
a) Anti-Sm positive patient sera.
b) Monoclonal anti-hexa-His-tag antibody.

Immunological functions

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels).

Safety Data Sheet

SDS

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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