SNRPD3 Human, Sf9

Small nuclear ribonucleoprotein D3 polypeptide 18kDa, Sm-D3, snRNP core protein D3.

SNRPD3 is a small nuclear ribonucleoprotein (snRNPs) that contains the spliceosome in eukaryotes. SNRPD3 is essential for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Alternative splicing happens in this locus and two transcript variants encoding the same protein were branded.

SNRPD3 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 14,739 Dalton.
SNRPD3 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.

Sf9 insect cells.

Sterile Filtered clear solution.

SNRPD3 is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
Avoid multiple freeze-thaw cycles.

Greater than 80% as determined by SDS-PAGE.

0.4-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Western blot with anti-Sm positive patient sera.

1. Binds IgG-type human auto-antibodies.
2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.