Catalogue number
PRO-065
Synonyms
U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4, YER112W.
Introduction
LSM4 (U6 snRNA-associated Sm-like protein) forms donut-shaped heptameric complexes which are involved in various steps of RNA metabolism. LSM4 facilitates RNA protein interactions and structural changes which are essential during ribosomal subunit assembly. LSM4 binds specifically to the 3'-terminal U-tract of U6 snRNA.
Description
LSM4 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 159 amino acids (1-139 a.a.) and having a molecular mass of 17.5kDa. The LSM4 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The LSM4 solution (0.5 mg/ml) 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRGR GGLQQQKQQK GRGMGGAGRG VFGGRGRGGI PGTGRGQPEK KPGRQAGKQ.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
