Catalogue number
PRO-2188
Synonyms
Zinc Finger, AN1-Type Domain 5, Zinc Finger Protein 216, ZNF216,
Zinc Finger A20 Domain-Containing Protein 2, Zinc Finger, A20 Domain Containing
2, ZA20D2, AN1-Type Zinc Finger Protein 5, ZFAND5A, AN1-type zinc finger
protein 5, Zinc finger A20 domain-containing protein 2, Zinc finger protein 216.
Introduction
Zinc Finger, AN1-Type Domain 5, also known as ZFAND5 is implicated in
protein degradation through the ubiquitin-proteasome system. ZFAND5 performs by
anchoring ubiquitinated proteins to the proteasome and takes part in
ubiquitin-mediated protein degradation during muscle atrophy. Furthemore,
ZFAND5 plays a role in the regulation of NF-kappa-B activation as well as
apoptosis. ZFAND5 inhibits NF-kappa-B activation as well as tumor necrosis
factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation.
Description
ZFAND5 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 236 amino acids (1-213 a.a) and having a molecular
mass of 25.5kDa (Molecular size on SDS-PAGE will appear higher).
ZFAND5 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
ZFAND5 protein solution (0.5mg/ml) containing 20mM Phosphate
buffer saline (pH 8.0), 30% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks.
Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM
SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT
EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP
YDYKAEAAAK IRKENPVVVA EKIQRI.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
