WB: This antibody yields stronger signals in Western blot experiments than cat. no. 135 511 but is less sensitive than cat. no. 135 011 and the polyclonal VGluT 1 antibodies.
IP: Coupling to protein A is recommended for IP, since covalent coupling to activated sepharose leads to considerable loss of activity.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 135 303 as detector antibody (protocol for sandwich-ELISA).VGLUT 1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
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