Uvitex 2B fluorescent dye will bind to the chitin in fungal walls and is highly selective for fungi and algae in tissue sections. It has a slower fade rate than more commonly used fungal fluorescent dyes.
(C.I. Fluorescent Brightener 362; Derivative of stilbene disulfonic acid.)
Commonly used protocol for staining of Microsporidia and Blastomyces is as follows:
Excitation Max: 350nm
Emission Max: 435nm in PBS Buffer
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