Trypan Blue 0.4% Solution

The dye exclusion test is used to determine the number of viable cells present in a cell suspension. It is based on the principle that live cells possess intact cell membranes that exclude certain dyes, such as trypan blue, Eosin, or propidium, whereas dead cells do not. In this test, a cell suspension is simply mixed with dye and then visually examined to determine whether cells take up or exclude dye. In the protocol presented here, a viable cell will have a clear cytoplasm whereas a nonviable cell will have a blue cytoplasm.

This product is for research use only and is not intended for use in humans or for in vitro diagnostic use.

MSDS / Technical Data Sheets / Product Literature

• msdsMaterial Safety Datasheet 25561 (PDF)
• dataData Sheet TDS 1005 Trypan Blue (PDF)

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