Zika virus NS1 antibody – clone B4 (Biotin conjugated)
Mouse anti Zika virus NS1 antibody is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus.
This mouse anti Zika virus NS1 antibody has been optimally conjugated to biotin, and is suitable for use in direct ELISA and in sandwich ELISA. Due to the hexameric nature of the NS1 antigen the B4 antibody will “pair” with itself in a sandwich assay. It may also be used as a “pair” with clone D11. This antibody has not been tested directly for use in immunofluorescence assays, but AbZIKVNS1-B4 (unconjugated Mouse Anti-Zika Virus NS1 Antibody) has been validated for immunofluorescence. Therefore, it is likely that conjugated antibody would perform in a similar manner.
The NS1 protein is a major non-strucutural protein expressed by the Zika Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Zika.
Zika virus is an emerging disease that is spread by Aedes mosquitoes. The virus was first isolated in Central Africa, and has since spread to South Asia and more recently to South America. It is a member of the flavivirus family, and is structurally closely related to viruses such as Dengue Fever Virus. Outbreaks were reported in Micronesia in 2007 and in Brazil in 2015, confirming at least 13 autochthonous infections. The Zika virus outbreak in Brazil in 2016 has gained world-wide attention, and has been linked to an increasing number of microcephaly cases. In April 2016 the Centers for Disease Control, in the USA, confirmed the link between Zika virus infection of the fetus with microcephaly.
Clinically Zika virus can cause mild fever, rash, myalgia, arthralgia and headaches, with one in four infected individuals being asymptomatic. Due to similar symptoms Zika virus infected individuals can easily be mis-diagnosed as a dengue infection and vice-versa. In addition, Zika virus has been implicated in causing microcephaly through transmission in utero. There is no vaccine or specific treatment available for Zika virus.
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