PCR Direct Kit without DNA Purification
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产品名称: PCR Direct Kit without DNA Purification
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产品展商: Ivy Fine Chemicals
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简单介绍
PCR Direct Kit without DNA Purification
PCR Direct Kit without DNA Purification
的详细介绍
PCR Direct Kit without DNA Purification (Catalog BP2310)
4x Protein Digestion Buffer (1 mL for 50 rxn),10x PCR Enhancer (0.3 mL for 100 rxn)
Ivy Fine Chemicals Corporation, 1879 Old Cuthbert Rd, Suite 23, Cherry Hill, NJ 08034
DNA purification through sodium iodide extraction, Qiagen column, magnetic beads or
phenol chloroform extraction has been absolutely required for a successful PCR
reaction. The complex procedures and DNA loss during binding, precipitation and
washing has caused significant variabilities among scientists and laboratories.
Here our optimized kit allows an efficient protein digestion and a simple gene
quantitation by PCR or real time PCR. No sample separation and purification is
necessary from our kit. No special Lab skills are required for a good PCR run.
Recommended Protein Digestion:
Note: (1) add Proteinase K (not included) to 4x Protein Digestion Buffer at 500 ug/mL final
concentration and use immediately; (2) heat top (e.g. Thermocycler) and tight seal are
recommended during protein digestion and Proteinase K inactivation steps to prevent
evaporation and liquid loss.
Standards: 40 uL Each DNA Standard, 20 uL of 40 mg/mL Surrogate Protein, 20 uL of
4x Protein Digestion Buffer containing 500 ug/mL Proteinase K, vortex and 55℃
overnight;
Samples: 40 uL of 20 mg/mL Each Sample, 20 uL of water (non-spiked) or DNA spike,
20 uL of 4x Protein Digestion Buffer containing 500 ug/mL Proteinase K, vortex and
55℃ overnight;
Inactivation of Proteinase K: heat standards and samples at 95℃for 5 min.
PCR or Real Time PCR: add 0.1 volume of 10 x PCR Enhancer to your routine PCR
or real time PCR reaction (e.g. 3 uL to 27 uL PCR reaction).
