Product Name
KCNE2, siRNA
Full Product Name
KCNE2 siRNA (Mouse)
Product Synonym Names
Potassium voltage-gated channel subfamily E member 2; MinK-related peptide 1; Minimum potassium ion channel-related peptide 1; Potassium channel subunit beta MiRP1
Product Gene Name
KCNE2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9D808
Specificity
KCNE2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KCNE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCNE2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCNE2 sirna
siRNA to inhibit KCNE2 expression using RNA interference
Applications Tested/Suitable for KCNE2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCNE2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_598871.1
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NCBI GenBank Nucleotide #
NM_134110.3
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UniProt Primary Accession #
Q9D808
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UniProt Secondary Accession #
Q8R1Z7[Other Products]
UniProt Related Accession #
Q9D808[Other Products]
Molecular Weight
14,370 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily E member 2
NCBI Official Synonym Full Names
potassium voltage-gated channel, Isk-related subfamily, gene 2
NCBI Official Symbol
Kcne2 [Similar Products]
NCBI Official Synonym Symbols
MiRP1; AW048273; 2200002I16Rik
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NCBI Protein Information
potassium voltage-gated channel subfamily E member 2
UniProt Protein Name
Potassium voltage-gated channel subfamily E member 2
UniProt Synonym Protein Names
MinK-related peptide 1; Minimum potassium ion channel-related peptide 1; Potassium channel subunit beta MiRP1
Protein Family
Potassium voltage-gated channel subfamily
UniProt Gene Name
Kcne2 [Similar Products]
UniProt Entry Name
KCNE2_MOUSE
UniProt Comments for KCNE2
KCNE2: Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Associated with KCNH2/HERG is proposed to form the rapidly activating component of the delayed rectifying potassium current in heart (IKr). May associate with KCNQ2 and/or KCNQ3 and modulate the native M-type current. May associate with KCNQ1/KVLTQ1 and elicit a voltage-independent current. May associate with HCN1 and HCN2 and increase potassium current. Defects in KCNE2 are the cause of long QT syndrome type 6 (LQT6). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. KCNE2 mutants form channels that open slowly and close rapidly, thereby diminishing potassium currents. Defects in KCNE2 are the cause of familial atrial fibrillation type 4 (ATFB4). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the potassium channel KCNE family.
Protein type: Membrane protein, integral
Cellular Component: voltage-gated potassium channel complex; cell surface; membrane; lysosome; integral to membrane; plasma membrane
Molecular Function: voltage-gated potassium channel activity; protein binding; potassium channel regulator activity; protein homodimerization activity; potassium channel activity; delayed rectifier potassium channel activity; voltage-gated ion channel activity; inward rectifier potassium channel activity
Biological Process: potassium ion import; transport; ion transport; potassium ion transport
Research Articles on KCNE2
1. Kcne2-deficient mice, in addition to the previously reported phenotypes, also present with iron-deficient anemia.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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