Product Name
KCNH2, siRNA
Full Product Name
KCNH2 siRNA (Human)
Product Synonym Names
ERG; ERG1; HERG; Potassium voltage-gated channel subfamily H member 2; Eag homolog; Ether-a-go-go-related gene potassium channel 1; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1; Voltage-gated potassium channel subunit Kv11.1
Product Gene Name
KCNH2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q12809
Specificity
KCNH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KCNH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of KCNH2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
KCNH2 sirna
siRNA to inhibit KCNH2 expression using RNA interference
Applications Tested/Suitable for KCNH2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for KCNH2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000229.1
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NCBI GenBank Nucleotide #
NM_000238.3
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UniProt Primary Accession #
Q12809
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UniProt Secondary Accession #
O75418; O75680; Q708S9; Q9BT72; Q9BUT7; Q9H3P0; A5H1P7; C4PFH9; D3DX04[Other Products]
UniProt Related Accession #
Q12809[Other Products]
Molecular Weight
115,636 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily H member 2 isoform a
NCBI Official Synonym Full Names
potassium channel, voltage gated eag related subfamily H, member 2
NCBI Official Symbol
KCNH2 [Similar Products]
NCBI Official Synonym Symbols
ERG1; HERG; LQT2; SQT1; ERG-1; H-ERG; HERG1; Kv11.1
[Similar Products]
NCBI Protein Information
potassium voltage-gated channel subfamily H member 2
UniProt Protein Name
Potassium voltage-gated channel subfamily H member 2
UniProt Synonym Protein Names
Eag homolog; Ether-a-go-go-related gene potassium channel 1; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1; Voltage-gated potassium channel subunit Kv11.1
Protein Family
Potassium voltage-gated channel subfamily
UniProt Gene Name
KCNH2 [Similar Products]
UniProt Synonym Gene Names
ERG; ERG1; HERG; ERG-1; Eag-related protein 1; Ether-a-go-go-related protein 1; H-ERG; hERG-1; hERG1 [Similar Products]
UniProt Entry Name
KCNH2_HUMAN
NCBI Summary for KCNH2
This gene encodes a voltage-activated potassium channel belonging to the eag family. It shares sequence similarity with the Drosophila ether-a-go-go (eag) gene. Mutations in this gene can cause long QT syndrome type 2 (LQT2). Transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Comments for KCNH2
Kv11.1: the ether-a-go-go related gene is a pore-forming (alpha) subunit of voltage-gated inwardly rectifying potassium channel is associated with cardiac arrhythmias and rhythmic excitability of the pituitary. Channel properties are modulated by cAMP and subunit assembly. Mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr). The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits. Heteromultimer with Kv11.2 and Kv11.3. Interacts with ALG10B. Heteromultimer with KCNE1 and KCNE2. Defects in Kv11.1 are the cause of long QT syndrome type 2 (LQT2), a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. Deafness is often associated with LQT2. Defects in Kv11.1 are the cause of short QT syndrome type 1 (SQT1), a heart disorder characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. Four isoforms of the human protein are produced by alternative splicing. Isoform 3 has no channel activity by itself, but modulates channel characteristics when associated with isoform 1.
Protein type: Motility/polarity/chemotaxis; Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: voltage-gated potassium channel complex; cell surface; cytoplasm; plasma membrane; nuclear envelope
Molecular Function: identical protein binding; voltage-gated potassium channel activity; protein binding; protein homodimerization activity; ubiquitin protein ligase binding; delayed rectifier potassium channel activity; inward rectifier potassium channel activity; two-component sensor activity
Biological Process: synaptic transmission; regulation of membrane potential; two-component signal transduction system (phosphorelay); regulation of the rate of heart contraction by hormone; potassium ion homeostasis; cardiac muscle contraction
Disease: Long Qt Syndrome 2; Short Qt Syndrome 1
Research Articles on KCNH2
1. Women with elevated BMI have enhanced hERG activity as a result of low beta-inhibitory protein expression, which likely contributes to weak contractions and poor labour outcomes.
Precautions
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