Product Name
BTD, Recombinant Protein
Full Product Name
BTD, 44-545aa, Human, His-tag, Baculovirus
Product Synonym Names
Biotinidase isoform 1; BTD; biotinidase
Product Gene Name
BTD recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
AHTGEESVAD HHEAEYYVAA VYEHPSILSL NPLALISRQE ALELMNQNLD IYEQQVMTAA QKDVQIIVFP EDGIHGFNFT RTSIYPFLDF MPSPQVVRWN PCLEPHRFND TEVLQRLSCM AIRGDMFLVA NLGTKEPCHS SDPRCPKDGR YQFNTNVVFS NNGTLVDRYR KHNLYFEAAF DVPLKVDLIT FDTPFAGRFG IFTCFDILFF DPAIRVLRDY KVKHVVYPTA WMNQLPLLAA IEIQKAFAVA FGINVLAANV HHPVLGMTGS GIHTPLESFW YHDMENPKSH LIIAQVAKNP VGLIGAENAT GETDPSHSKF LKILSGDPYC EKDAQEVHCD EATKWNVNAP PTFHSEMMYD NFTLVPVWGK EGYLHVCSNG LCCYLLYERP TLSKELYALG VFDGLHTVHG TYYIQVCALV RCGGLGFDTC GQEITEATGI FEFHLWGNFS TSYIFPLFLT SGMTLEVPDQ LGWENDHYFL RKSRLSSGLV TAALYGRLYE RDLEHHHHHH
3D Structure
ModBase 3D Structure for P43251
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (lot specific)
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Other Notes
Small volumes of BTD recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
BTD recombinant protein
BTD, also known as biotinidase isoform 1, is a member of the nitrilase superfamily, which consists of 12 families of nitrilases, amidases, carbamylases, and N-acyltrasferases. It catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to biotin and lysine. It may have an important regulatory role in chromatin/DNA function. Recombinant human BTD, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for BTD recombinant protein
Signal Transduction; Metabolism
Applications Tested/Suitable for BTD recombinant protein
SDS-PAGE
SDS-Page of BTD recombinant protein
NCBI/Uniprot data below describe general gene information for BTD. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001268652
[Other Products]
NCBI GenBank Nucleotide #
NM_001281723.2
[Other Products]
UniProt Primary Accession #
P43251
[Other Products]
UniProt Secondary Accession #
Q96EM9; A6NHF2; B2R865; B4DFX1; B4DLJ9; B7Z7C9; F8W1Q3[Other Products]
UniProt Related Accession #
P43251[Other Products]
Molecular Weight
57.8kDa (510aa) 50-70kDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
biotinidase isoform 1
NCBI Official Synonym Full Names
biotinidase
NCBI Official Symbol
BTD [Similar Products]
NCBI Protein Information
biotinidase
UniProt Protein Name
Biotinidase
Protein Family
Biotinidase
UniProt Gene Name
BTD [Similar Products]
UniProt Synonym Gene Names
Biotinase [Similar Products]
NCBI Summary for BTD
The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
UniProt Comments for BTD
Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation.
Product References and Citations for BTD recombinant protein
Hymes J., et al. (2001) Hum Mutat. 18:375-381.; Cole H., et al. (1994) J Biol Chem. 269:6566-6570.
Research Articles on BTD
1. Biotinidase deficiency is reviewed.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
