Acid Sphingomyelinase, ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of ASM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS734434 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acid Sphingomyelinase (ASM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASM. The ELISA analytical biochemical technique of the MBS734434 kit is based on ASM antibody-ASM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: ASM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ASM antibody and an ASM-HRP conjugate. The assay sample and buffer are incubated together with ASM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASM concentration since ASM from samples and ASM-HRP conjugate compete for the anti-ASM antibody . Since the number of sites is limited, as more sites are occupied by ASM from the sample, fewer sites are left to bind ASM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASM concentration in each sample is interpolated from this standard curve.

Cell Biology

69,624 Da

sphingomyelin phosphodiesterase 1, acid lysosomal

sphingomyelin phosphodiesterase; acid sphingomyelinase

Sphingomyelin phosphodiesterase

ASM; aSMase  [Similar Products]

ASM_HUMAN

The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]

SMPD1: Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. Monomer. Belongs to the acid sphingomyelinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phosphodiesterase; EC 3.1.4.12; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 11p15.4-p15.1

Cellular Component: lysosomal lumen; extracellular space; lysosome; plasma membrane; endosome; lamellar body

Molecular Function: protein binding; sphingomyelin phosphodiesterase activity; hydrolase activity, acting on glycosyl bonds

Biological Process: response to drug; nervous system development; negative regulation of MAP kinase activity; sphingolipid metabolic process; sphingomyelin metabolic process; positive regulation of apoptosis; ceramide biosynthetic process; glycosphingolipid metabolic process; sphingomyelin catabolic process; signal transduction; response to cocaine; positive regulation of protein amino acid dephosphorylation

Disease: Niemann-pick Disease, Type B; Niemann-pick Disease, Type A

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