Product Name
CYB5R3, siRNA
Full Product Name
CYB5R3 siRNA (Mouse)
Product Synonym Names
DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1
Product Gene Name
CYB5R3 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9DCN2
Specificity
CYB5R3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYB5R3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CYB5R3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CYB5R3 sirna
siRNA to inhibit CYB5R3 expression using RNA interference
Applications Tested/Suitable for CYB5R3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CYB5R3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_084063.1
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NCBI GenBank Nucleotide #
NM_029787.2
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UniProt Primary Accession #
Q9DCN2
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UniProt Related Accession #
Q9DCN2[Other Products]
Molecular Weight
31,550 Da
NCBI Official Full Name
NADH-cytochrome b5 reductase 3
NCBI Official Synonym Full Names
cytochrome b5 reductase 3
NCBI Official Symbol
Cyb5r3 [Similar Products]
NCBI Official Synonym Symbols
Dia1; Dia-1; C85115; WU:Cyb5r3; 0610016L08Rik; 2500002N19Rik; WU:AL591952.1-001; WU:AL591952.1-002; WU:AL591952.1-003
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NCBI Protein Information
NADH-cytochrome b5 reductase 3
UniProt Protein Name
NADH-cytochrome b5 reductase 3
UniProt Synonym Protein Names
Diaphorase-1
Protein Family
NADH-cytochrome b5 reductase
UniProt Gene Name
Cyb5r3 [Similar Products]
UniProt Synonym Gene Names
Dia1; B5R; Cytochrome b5 reductase [Similar Products]
UniProt Entry Name
NB5R3_MOUSE
UniProt Comments for CYB5R3
CYB5R3: Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Defects in CYB5R3 are the cause of methemoglobinemia CYB5R3-related (METHB-CYB5R3). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. There are two types of methemoglobinemia CYB5R3-related. In type 1, the defect affects the soluble form of the enzyme, is restricted to red blood cells, and causes well- tolerated methemoglobinemia. In type 2, the defect affects both the soluble and microsomal forms of the enzyme and is thus generalized, affecting red cells, leukocytes and all body tissues. Type 2 methemoglobinemia is associated with mental deficiency and other neurologic symptoms. Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. 3 isoforms of the human protein are produced by alternative promoter.
Protein type: Oxidoreductase; Mitochondrial; EC 1.6.2.2; Carbohydrate Metabolism - amino sugar and nucleotide sugar
Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; membrane; endoplasmic reticulum; cytoplasm; mitochondrial inner membrane
Molecular Function: protein binding; FAD binding; cytochrome-b5 reductase activity; oxidoreductase activity; ADP binding; NAD binding; AMP binding
Biological Process: steroid metabolic process; cholesterol metabolic process; sterol biosynthetic process; lipid metabolic process; cholesterol biosynthetic process; steroid biosynthetic process
Research Articles on CYB5R3
1. Amino and carboxy terminal fragments of mDia1 form a tetrameric complex composed of two interlocking N+C dimers.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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