Nucleoporin GLE1 (GLE1), ELISA Kit

GLE1L; LCCS; LCCS1; hGLE1; GLE1 RNA export mediator homolog; GLE1 RNA export mediator-like; GLE1-like; RNA export mediator

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Rat GLE1. No significant cross-reactivity or interference between Rat GLE1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of GLE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS287631 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nucleoporin GLE1 (GLE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GLE1. The ELISA analytical biochemical technique of the MBS287631 kit is based on GLE1 antibody-GLE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GLE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GLE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Description: This assay employs a two-site sandwich ELISA to quantitate GLE1 in samples. An antibody specific for GLE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GLE1Lencodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export.
Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

79,492 Da

GLE1 RNA export mediator

nucleoporin GLE1

Nucleoporin GLE1

Gle1l  [Similar Products]

Required for the export of mRNAs containing poly(A) tails from the nucleus into the cytoplasm. May be involved in the terminal step of the mRNA transport through the nuclear pore complex (NPC) ().

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