Product Name
5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A), Cell Line
Full Product Name
Human 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A) ACTOne Stable Cell Line
Product Gene Name
HTR1A cell line
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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Cell Culture Medium
Growth medium (for HTR1A Stable Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418, 1 ug/ml Puromycin
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Mycoplasma Contamination Test
This cell line has been tested negative for Mycoplasma sp.
Shipping Note
Product available only in the USA.
Growth Properties
Adherent
Detection
FlexStation or Microplate reader
Other Notes
Small volumes of HTR1A cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HTR1A cell line
Introduction: HTR1A, expressed in the pancreas and gastrointestinal tract, encodes a member of the rhodopsin subfamily of G-proteincoupled receptors. The encoded protein is activated by lipid amides including lysophosphatidylcholine and oleoylethanolamide and may be involved in glucose homeostasis. This protein is a potential drug target in the treatment of type 2 diabetes.
Description: Human HTR1A ACTOne is a HEK293-CNG cell line that expresses recombinant human HTR1A. HEK293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye (Cat# MBS430187). The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Product Categories/Family for HTR1A cell line
Cell Based GPCR-PDE Compound Assay System; ACTOne Assay on G-Protein Coupled Receptors (GPCRs); ACTOne GPCR Stable Cell Lines; 5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A) ACTOne Stable Cell Line
Application Notes for HTR1A cell line
cAMP dependent human HTR1A cell-based assay
cell-based high-throughput screening of human HTR1A agonists/antagonists.
Testing Data of HTR1A cell line
Testing Data of HTR1A cell line
NCBI/Uniprot data below describe general gene information for HTR1A. It may not necessarily be applicable to this product.
NCBI Accession #
XP_003692
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NCBI GenBank Nucleotide #
XM_003692.2
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UniProt Secondary Accession #
Q6LAE7[Other Products]
UniProt Related Accession #
P08908[Other Products]
Molecular Weight
46,107 Da
NCBI Official Synonym Full Names
5-hydroxytryptamine receptor 1A
NCBI Official Symbol
HTR1A [Similar Products]
NCBI Official Synonym Symbols
G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
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NCBI Protein Information
5-hydroxytryptamine receptor 1A
UniProt Protein Name
5-hydroxytryptamine receptor 1A
UniProt Synonym Protein Names
G-21; Serotonin receptor 1A
Protein Family
5-hydroxytryptamine receptor
UniProt Gene Name
HTR1A [Similar Products]
UniProt Synonym Gene Names
ADRB2RL1; ADRBRL1; 5-HT-1A; 5-HT1A [Similar Products]
NCBI Summary for HTR1A
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]
UniProt Comments for HTR1A
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.
Research Articles on HTR1A
1. The results suggest that HTR1A/1B DNA hypomethylation and its interaction with recent life stress might drive impaired antidepressant treatment response. Meanwhile, DNA methylation, in turn, was modified by antidepressant treatment and environments.
Precautions
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