Full Product Name
DAP12 Antibody
Product Synonym Names
DAP-12; DAP 12; DAP12; TYRO protein tyrosine kinase binding protein; TYROBP
Product Gene Name
anti-DAP12 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O54885
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) affinity purified rabbit anti-DAP12 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Appearance: Colorless liquid
Concentration
0.5 mg/ml (lot specific)
Immunogen
Synthetic peptide corresponding to residues surrounding amino acids 94 of mouse DAP-12
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Other Notes
Small volumes of anti-DAP12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-DAP12 antibody
Background: DAP12 (KARAP) is a natural killer (NK) cell-expressed cell surface receptor and belongs to the immunoglobulin and C-type lectin superfamily. Unlike other members of this family which possess immunoreceptor tyrosine-based inhibitory motifs (ITIM), DAP12 contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and is present as a disulphide-bonded homodimer. Crosslinking of DAP 12-KIR complexes (membrane glycoproteins of the killer-cell inhibitory receptor family without an ITIM in their cytoplasmic domain) results in cellular activation.
Applications Tested/Suitable for anti-DAP12 antibody
Western Blot (WB)
Application Notes for anti-DAP12 antibody
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
Testing Data of anti-DAP12 antibody
NCBI/Uniprot data below describe general gene information for DAP12. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035792.1
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NCBI GenBank Nucleotide #
NM_011662.2
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UniProt Primary Accession #
O54885
[Other Products]
UniProt Secondary Accession #
O88603[Other Products]
UniProt Related Accession #
O54885[Other Products]
Molecular Weight
12,367 Da
NCBI Official Full Name
TYRO protein tyrosine kinase-binding protein
NCBI Official Synonym Full Names
TYRO protein tyrosine kinase binding protein
NCBI Official Symbol
Tyrobp [Similar Products]
NCBI Official Synonym Symbols
Ly83; DAP12; KARAP
[Similar Products]
NCBI Protein Information
TYRO protein tyrosine kinase-binding protein
UniProt Protein Name
TYRO protein tyrosine kinase-binding protein
UniProt Synonym Protein Names
DNAX-activation protein 12; Killer-activating receptor-associated protein; KAR-associated protein
UniProt Gene Name
Tyrobp [Similar Products]
UniProt Synonym Gene Names
Dap12; Karap; KAR-associated protein [Similar Products]
UniProt Entry Name
TYOBP_MOUSE
UniProt Comments for DAP12
TYROBP: Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. Involved for instance in neutrophil activation mediated by integrin. Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. Belongs to the TYROBP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Cell surface
Cellular Component: cell surface; membrane; integral to membrane
Molecular Function: identical protein binding; protein binding; receptor binding
Biological Process: integrin-mediated signaling pathway; neutrophil activation during immune response; macrophage activation during immune response
Research Articles on DAP12
1. a previously unrecognized role for DAP12 expression in tissue-resident alveolar macrophages in mediating acute noninfectious tissue injury through regulation of neutrophil trafficking.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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