Product Name
MCFD2, siRNA
Full Product Name
MCFD2 siRNA (Human)
Product Synonym Names
SDNSF; Multiple coagulation factor deficiency protein 2; Neural stem cell-derived neuronal survival protein
Product Gene Name
MCFD2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q8NI22
Specificity
MCFD2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MCFD2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of MCFD2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MCFD2 sirna
siRNA to inhibit MCFD2 expression using RNA interference
Applications Tested/Suitable for MCFD2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for MCFD2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001164977.1
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NCBI GenBank Nucleotide #
NM_001171506.2
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UniProt Primary Accession #
Q8NI22
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UniProt Secondary Accession #
Q53SS3; Q68D61; Q8N3M5; A8K7W2; D6W5A9; E9PD95[Other Products]
UniProt Related Accession #
Q8NI22[Other Products]
Molecular Weight
14,431 Da
NCBI Official Full Name
multiple coagulation factor deficiency protein 2 isoform A
NCBI Official Synonym Full Names
multiple coagulation factor deficiency 2
NCBI Official Symbol
MCFD2 [Similar Products]
NCBI Official Synonym Symbols
F5F8D; SDNSF; F5F8D2; LMAN1IP
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NCBI Protein Information
multiple coagulation factor deficiency protein 2
UniProt Protein Name
Multiple coagulation factor deficiency protein 2
UniProt Synonym Protein Names
Neural stem cell-derived neuronal survival protein
Protein Family
Multiple coagulation factor deficiency protein
UniProt Gene Name
MCFD2 [Similar Products]
UniProt Synonym Gene Names
SDNSF [Similar Products]
UniProt Entry Name
MCFD2_HUMAN
NCBI Summary for MCFD2
This gene encodes a soluble luminal protein with two calmodulin-like EF-hand motifs at its C-terminus. This protein forms a complex with LAMN1 (lectin mannose binding protein 1; also known as ERGIC-53) that facilitates the transport of coagulation factors V (FV) and VIII (FVIII) from the endoplasmic reticulum to the Golgi apparatus via an endoplasmic reticulum Golgi intermediate compartment (ERGIC). Mutations in this gene cause combined deficiency of FV and FVIII (F5F8D); a rare autosomal recessive bleeding disorder characterized by mild to moderate bleeding and coordinate reduction in plasma FV and FVIII levels. This protein has also been shown to maintain stem cell potential in ***** central nervous system and is a marker for testicular germ cell tumors. The 3' UTR of this gene contains a transposon-like human repeat element named 'THE 1'. A processed RNA pseudogene of this gene is on chromosome 6p22.1. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jun 2010]
UniProt Comments for MCFD2
MCFD2: The MCFD2-LMAN1 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. Plays a role in the secretion of coagulation factors. Defects in MCFD2 are a cause of factor V and factor VIII combined deficiency type 2 (F5F8D2); also known as multiple coagulation factor deficiency 2 (MCFD2). F5F8D2 is a blood coagulation disorder characterized by bleeding symptoms similar to those in hemophilia or parahemophilia, that are caused by single deficiency of FV or FVIII, respectively. The most common symptoms are epistaxis, menorrhagia, and excessive bleeding during or after trauma. Plasma levels of coagulation factors V and VIII are in the range of 5 to 30% of normal. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: Golgi apparatus; endoplasmic reticulum; ER-Golgi intermediate compartment
Molecular Function: calcium ion binding
Biological Process: vesicle-mediated transport; protein transport; cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Disease: Factor V And Factor Viii, Combined Deficiency Of, 2
Research Articles on MCFD2
1. A novel missense mutation, namely Asp81Ala in exon 3 of MCFD2 gene, is firstly reported and described as a cause of combined FV and FVIII deficiency in a Chinese family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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