TREM-2b, Monoclonal Antibody

Anti-mouse Triggering Receptor Expressed on Myeloid Cells 2b [TREM-2b] (#3C66)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 17; NC_000083.6 (48346401..48352276). Location: 17 C; 17

Monoclonal

IgG2

(5J46)

Rat

This antibody detects mouse of TREM-2b, but not TREM-1 in Western blotting.

Protein A/G affinity chromatography

The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 um filtered solution in phosphate-buffered saline (PBS). Lyophilized samples are stable for 2 years from date of receipt when stored at

Lyophilized samples are stable for 2 years from date of receipt when stored at -70 degree C. Reconstituted antibody can be aliquoted and stored frozen at < -20 degree C for at least for six months without detectable loss of activity.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-TREM-2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

TREM2 is a single Ig domain receptor expressed by cells of myeloid lineage. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. TREM2 is a pattern recognition receptor that binds anionic ligands. It associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses.

Western Blot (WB)

1. Western blotting: Yes (1:500-1000)

24,527 Da

triggering receptor expressed on myeloid cells 2

triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c

Triggering receptor expressed on myeloid cells 2

Trem2a; Trem2b; Trem2c; TREM-2  [Similar Products]

TREM2_MOUSE

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Function: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Ref.2

Subunit structure: Interacts with TYROBP/DAP12. Ref.2

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein

Potential. Isoform 2: Secreted

Potential.

Tissue specificity: Expressed at higher levels in the CNS, heart and lung than in lymph nodes or in other non-lymphoid tissues such as kidney, liver and testis. In the CNS not all microglia express TREM2. Brain regions with an incomplete blood-brain barrier had the lowest percentages of TREM2 expressing microglia, whereas the lateral entorhinal and cingulate cortex had the highest percentages. Ref.2 Ref.3

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution: The sequence AAF69825.1 differs from that shown. Reason: Frameshift at positions 70 and 78.

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