Product Name
TREM2, siRNA
Full Product Name
TREM2 siRNA (Mouse)
Product Synonym Names
TREM2A; TREM2B; TREM2C; Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2
Product Gene Name
TREM2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q99NH8
Specificity
TREM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TREM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TREM2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TREM2 sirna
siRNA to inhibit TREM2 expression using RNA interference
Applications Tested/Suitable for TREM2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TREM2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001259007.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001272078.1
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UniProt Primary Accession #
Q99NH8
[Other Products]
UniProt Secondary Accession #
Q8CGK4; Q8CIA6; Q99NH9; Q9JL34[Other Products]
UniProt Related Accession #
Q99NH8[Other Products]
Molecular Weight
27,304 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 2 isoform 2
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol
Trem2 [Similar Products]
NCBI Official Synonym Symbols
TREM-2; Trem2a; Trem2b; Trem2c
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NCBI Protein Information
triggering receptor expressed on myeloid cells 2
UniProt Protein Name
Triggering receptor expressed on myeloid cells 2
UniProt Synonym Protein Names
Triggering receptor expressed on monocytes 2
Protein Family
Triggering receptor expressed on myeloid cells
UniProt Gene Name
Trem2 [Similar Products]
UniProt Synonym Gene Names
Trem2a; Trem2b; Trem2c; TREM-2 [Similar Products]
UniProt Entry Name
TREM2_MOUSE
NCBI Summary for TREM2
The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
UniProt Comments for TREM2
TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; extracellular region
Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; lipopolysaccharide binding
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; lipopolysaccharide-mediated signaling pathway; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of antigen processing and presentation of peptide antigen via MHC class II
Research Articles on TREM2
1. Corpus callosum microglia normally expand with age, but aged Trem2(-/-) mice had fewer microglia with an abnormal morphology which failed to amplify transcripts indicative of activation, phagocytosis, and lipid catabolism in response to myelin damage.
Precautions
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Disclaimer
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