GTF2I, Polyclonal Antibody

General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 protein; GTF2I; BAP135; WBSCR6

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the N-terminal region of human GTF2I.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.4 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-GTF2I antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a multifunctional phosphoprotein with
roles in transcription and signal transduction. It is deleted in
Williams-Beuren syndrome, a multisystem developmental disorder
caused by the deletion of contiguous genes at chromosome 7q11.23.
Alternative splicing results in multiple transcript variants.
Related pseudogenes have been identified on chromosomes 7, 13 and
21.

Crown Antibodies

Western Blot (WB), ELISA (EIA), Immunofluorescence (IF)

IF~~1:10~50
WB~~1:1000

Fluorescent image of Hela cell stained with GTF2I Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GTF2I primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). GTF2I immunoreactivity is localized to Nucleus significantly.

GTF2I Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).

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general transcription factor IIi

general transcription factor II-I

General transcription factor II-I

BAP135; WBSCR6; GTFII-I; TFII-I; BAP-135; BTK-associated protein 135; SPIN  [Similar Products]

GTF2I_HUMAN

This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]

GTF2I: a multifunctional phosphoprotein with roles in transcription and signal transduction. Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and Phox1 and interacts cooperatively with Phox1 to promote serum-inducible transcription of a reporter gene driven by the C- FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Its gene is known to contain at least 34 exons. Four splice variant isoforms have been described.

Protein type: Transcription, coactivator/corepressor; DNA-binding

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; cell projection; membrane; cell soma; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; mitogen-activated protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of angiogenesis; regulation of transcription, DNA-dependent; reduction of cytosolic calcium ion concentration; transition between slow and fast fiber; signal transduction

Disease: Williams-beuren Syndrome

Antonell, A., et al. J. Med. Genet. 47(5):312-320(2010)
Lazebnik, M.B., et al. J. Biol. Chem. 284(52):36234-36239(2009)
Sacristan, C., et al. Eur. J. Immunol. 39(9):2584-2595(2009)
Makeyev, A.V., et al. Gene 433 (1-2), 16-25 (2009) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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