Product Name
CHRM3, siRNA
Full Product Name
CHRM3 siRNA (Human)
Product Synonym Names
Muscarinic acetylcholine receptor M3
Product Gene Name
CHRM3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P20309
Specificity
CHRM3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CHRM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHRM3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHRM3 sirna
siRNA to inhibit CHRM3 expression using RNA interference
Applications Tested/Suitable for CHRM3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHRM3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000731.1
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NCBI GenBank Nucleotide #
NM_000740.2
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UniProt Primary Accession #
P20309
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UniProt Secondary Accession #
Q0VAJ8; Q4QRI3; Q5VXY2; Q9HB60[Other Products]
UniProt Related Accession #
P20309[Other Products]
Molecular Weight
66,128 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M3
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 3
NCBI Official Symbol
CHRM3 [Similar Products]
NCBI Official Synonym Symbols
HM3; PBS; EGBRS
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NCBI Protein Information
muscarinic acetylcholine receptor M3
UniProt Protein Name
Muscarinic acetylcholine receptor M3
Protein Family
Muscarinic acetylcholine receptor
UniProt Gene Name
CHRM3 [Similar Products]
UniProt Entry Name
ACM3_HUMAN
NCBI Summary for CHRM3
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]
UniProt Comments for CHRM3
mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
Protein type: Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q43
Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction
Molecular Function: protein binding; drug binding; receptor activity; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; nervous system development; cell proliferation; smooth muscle contraction; acetylcholine receptor signaling, muscarinic pathway; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion
Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism
Research Articles on CHRM3
1. an incorrect organizational structure of many mutants of hM3R provides the molecular basis for why they are poorly expressed and fail to be effectively trafficked to the cell surface.
Precautions
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Disclaimer
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