Product Name
CHRM3, siRNA
Full Product Name
CHRM3 siRNA (Mouse)
Product Synonym Names
CHRM-3; Muscarinic acetylcholine receptor M3; Mm3 mAChR
Product Gene Name
CHRM3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9ERZ3
Specificity
CHRM3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHRM3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of CHRM3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CHRM3 sirna
siRNA to inhibit CHRM3 expression using RNA interference
Applications Tested/Suitable for CHRM3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for CHRM3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_150372.1
[Other Products]
NCBI GenBank Nucleotide #
NM_033269.4
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UniProt Primary Accession #
Q9ERZ3
[Other Products]
UniProt Secondary Accession #
Q64055[Other Products]
UniProt Related Accession #
Q9ERZ3[Other Products]
Molecular Weight
66,212 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M3
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 3, cardiac
NCBI Official Symbol
Chrm3 [Similar Products]
NCBI Official Synonym Symbols
M3; M3R; Chrm-3
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NCBI Protein Information
muscarinic acetylcholine receptor M3
UniProt Protein Name
Muscarinic acetylcholine receptor M3
UniProt Synonym Protein Names
Mm3 mAChR
Protein Family
Muscarinic acetylcholine receptor
UniProt Gene Name
Chrm3 [Similar Products]
UniProt Synonym Gene Names
Chrm-3 [Similar Products]
UniProt Entry Name
ACM3_MOUSE
UniProt Comments for CHRM3
mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.
Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR
Cellular Component: asymmetric synapse; postsynaptic membrane; membrane; integral to plasma membrane; dendrite; integral to membrane; plasma membrane; synapse; nerve terminal; cell junction
Molecular Function: G-protein coupled receptor activity; signal transducer activity; acetylcholine receptor activity; drug binding; acetylcholine binding; G-protein coupled acetylcholine receptor activity
Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; acetylcholine receptor signaling, muscarinic pathway; smooth muscle contraction; regulation of vasoconstriction; digestion; regulation of vascular smooth muscle contraction; positive regulation of smooth muscle contraction; signal transduction; saliva secretion; muscarinic acetylcholine receptor, phospholipase C activating pathway; synaptic transmission, cholinergic
Research Articles on CHRM3
1. These findings indicate that the M(3) receptor on structural cells plays a proinflammatory role in CS-induced neutrophilic inflammation, whereas the M(3) receptor on inflammatory cells does not.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
