17-keto-steroid (17-KS), ELISA Kit

For Research Use Only. Not for use in diagnostic procedures.

Store all reagents at 2-8 degree C.

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Small volumes of 17-KS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS261437 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 17-keto-steroid (17-KS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing 17-KS. The ELISA analytical biochemical technique of the MBS261437 kit is based on 17-KS antibody-17-KS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect 17-KS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, 17-KS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

34,330 Da

hydroxysteroid (17-beta) dehydrogenase 3

testosterone 17-beta-dehydrogenase 3; 17-beta-HSD 3; 17(beta)HSD type 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase

Testosterone 17-beta-dehydrogenase 3

Edh17b3; 17-beta-HSD 3  [Similar Products]

DHB3_MOUSE

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.64

Cellular Component: intracellular membrane-bound organelle

Molecular Function: estradiol 17-beta-dehydrogenase activity; testosterone 17-beta-dehydrogenase (NADP+) activity; oxidoreductase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity

Biological Process: metabolic process; lipid metabolic process; steroid biosynthetic process

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