HSD17B3, Polyclonal Antibody

Testosterone 17-beta-dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase; HSD17B3; EDH17B3

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit Ig

Rabbit

This HSD17B3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human HSD17B3.

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5 (lot specific)

Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Small volumes of anti-HSD17B3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Cancer; Cell Biology; Metabolism; Signal Transduction

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

WB~~1:1000

Western blot analysis of HSD17B3 Antibody (Center) in K562 cell line lysates (35ug/lane). HSD17B3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human testis tissue reacted with HSD17B3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

HSD17B3 Antibody (Center) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

34516

hydroxysteroid (17-beta) dehydrogenase 3

testosterone 17-beta-dehydrogenase 3

Testosterone 17-beta-dehydrogenase 3

EDH17B3; SDR12C2; 17-beta-HSD 3  [Similar Products]

DHB3_HUMAN

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]

HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: Oxidoreductase; EC 1.1.1.64; Lipid Metabolism - androgen and estrogen

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: testosterone 17-beta-dehydrogenase (NADP+) activity

Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process

Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency

Li, J., et al. Breast Cancer Res. 12 (2), R19 (2010) :
Sata, F., et al. J Sex Med (2010) In press :
Ahn, J., et al. Hum. Mol. Genet. 18(19):3749-3757(2009)
Chakrabarti, B., et al. Autism Res 2(3):157-177(2009)
Beuten, J., et al. Cancer Epidemiol. Biomarkers Prev. 18(6):1869-1880(2009)
Andersson, S., et al. J. Clin. Endocrinol. Metab. 81(1):130-136(1996)

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