Product Name
HSD17B3, siRNA
Full Product Name
HSD17B3 siRNA (Rat)
Product Synonym Names
EDH17B3; Testosterone 17-beta-dehydrogenase 3; 17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
Product Gene Name
HSD17B3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O54939
Specificity
HSD17B3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HSD17B3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of HSD17B3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
HSD17B3 sirna
siRNA to inhibit HSD17B3 expression using RNA interference
Applications Tested/Suitable for HSD17B3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for HSD17B3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_446459.1
[Other Products]
NCBI GenBank Nucleotide #
NM_054007.1
[Other Products]
UniProt Primary Accession #
O54939
[Other Products]
UniProt Related Accession #
O54939[Other Products]
Molecular Weight
34,223 Da
NCBI Official Full Name
testosterone 17-beta-dehydrogenase 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
Hsd17b3 [Similar Products]
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
Protein Family
Testosterone 17-beta-dehydrogenase
UniProt Gene Name
Hsd17b3 [Similar Products]
UniProt Synonym Gene Names
Edh17b3; 17-beta-HSD 3 [Similar Products]
UniProt Entry Name
DHB3_RAT
NCBI Summary for HSD17B3
catalyzes the final step of testosterone biosynthesis in the testis [RGD, Feb 2006]
UniProt Comments for HSD17B3
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Protein type: Oxidoreductase; Lipid Metabolism - androgen and estrogen; EC 1.1.1.64
Cellular Component: intracellular membrane-bound organelle
Molecular Function: estradiol 17-beta-dehydrogenase activity; testosterone 17-beta-dehydrogenase (NADP+) activity; oxidoreductase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity
Biological Process: response to nutrient levels; response to metal ion; insecticide metabolic process; response to arsenic; hippocampus development; response to herbicide; response to insecticide; response to L-ascorbic acid; response to organic cyclic substance; cellular response to hormone stimulus; organic acid metabolic process; response to vitamin E; Leydig cell differentiation; response to cadmium ion; response to ethanol; biphenyl metabolic process; response to estrogen stimulus; response to steroid hormone stimulus; response to lead ion; response to activity; steroid biosynthetic process; response to corticosterone stimulus; phenol metabolic process
Research Articles on HSD17B3
1. 11beta-HSD1 is enzymatically coupled to 17beta-HSD3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
