Full Product Name
Brm Antibody
Product Gene Name
anti-Brm antibody
[Similar Products]
Product Synonym Gene Name
SMARCA2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P51531
Species Reactivity
Human, Mouse, Rat
Specificity
Brm antibody detects endogenous levels of Brm.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (lot specific)
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human Brm.
Immunogen Range
1328-1377
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-Brm antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Brm antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-Brm antibody
WB: 1:500~1:1000
ELISA: 1:10000
Western Blot (WB) of anti-Brm antibody
Western blot analysis of lysates from K562 cells, using Brm antibody.
NCBI/Uniprot data below describe general gene information for Brm. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001276325.1
[Other Products]
NCBI GenBank Nucleotide #
NM_001289396.1
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UniProt Primary Accession #
P51531
[Other Products]
UniProt Secondary Accession #
B1ALG3; B1ALG4; D3DRH4; D3DRH5[Other Products]
UniProt Related Accession #
P51531[Other Products]
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform a
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
SMARCA2 [Similar Products]
NCBI Official Synonym Symbols
BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
[Similar Products]
NCBI Protein Information
probable global transcription activator SNF2L2
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Synonym Protein Names
ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Protein Family
ATP-dependent helicase
UniProt Gene Name
SMARCA2 [Similar Products]
UniProt Synonym Gene Names
BAF190B; hBRM [Similar Products]
NCBI Summary for Brm
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]
UniProt Comments for Brm
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their ***** state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth ().
Research Articles on Brm
1. Two BRM promoter polymorphisms were strongly associated with hepatocellular carcinoma (HCC) prognosis but were not associated with increased HCC susceptibility.
Precautions
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