Product Name
HSD17B3, Polyclonal Antibody
Full Product Name
Goat anti-HSD17B3 (aa141-155) Antibody
Product Synonym Names
HSD17B3; hydroxysteroid (17-beta) dehydrogenase 3; EDH17B3; SDR12C2; 17-beta-HSD 3; 17-beta-HSD3; 17-beta-hydroxysteroid dehydrogenase type 3; short chain dehydrogenase/reductase family 12C, member 2; testicular 17-beta-hydroxysteroid dehydrogenase; testo; HSD17B3 antibody; hydroxysteroid (17-beta) dehydrogenase 3 antibody; EDH17B3 antibody; SDR12C2 antibody; 17-beta-HSD 3 antibody; 17-beta-HSD3 antibody; 17-beta-hydroxysteroid dehydrogenase type 3 antibody; short chain dehydrogenase/reductase family 12C; member 2 antibody; testicular 17-beta-hydroxysteroid dehydrogenase antibody; testo antibody; HSD17B3 (aa141-155)
Product Gene Name
anti-HSD17B3 antibody
[Similar Products]
Product Synonym Gene Name
HSD17B3[Similar Products]
Antibody/Peptide Pairs
HSD17B3 peptide (MBS428733) is used for blocking the activity of HSD17B3 antibody (MBS423643)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
SHFLNAPDEI QSLIH
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-SHFLNAPDEIQSLIH, from the internal region of the protein sequence according to NP_000188.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HSD17B3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HSD17B3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSD17B3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 33kDa band observed in Human Testis lysates (calculated MW of 34.5kDa according to NP_000188.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB) of anti-HSD17B3 antibody
(2ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NCBI/Uniprot data below describe general gene information for HSD17B3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000188.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000197.1
[Other Products]
UniProt Secondary Accession #
Q5U0Q6[Other Products]
UniProt Related Accession #
P37058[Other Products]
Molecular Weight
29,109 Da
NCBI Official Full Name
testosterone 17-beta-dehydrogenase 3
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol
HSD17B3 [Similar Products]
NCBI Official Synonym Symbols
EDH17B3; SDR12C2
[Similar Products]
NCBI Protein Information
testosterone 17-beta-dehydrogenase 3
UniProt Protein Name
Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Short chain dehydrogenase/reductase family 12C member 2; Testicular 17-beta-hydroxysteroid dehydrogenase
Protein Family
Testosterone 17-beta-dehydrogenase
UniProt Gene Name
HSD17B3 [Similar Products]
UniProt Synonym Gene Names
EDH17B3; SDR12C2; 17-beta-HSD 3 [Similar Products]
UniProt Entry Name
DHB3_HUMAN
NCBI Summary for HSD17B3
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
UniProt Comments for HSD17B3
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Protein type: EC 1.1.1.64; Lipid Metabolism - androgen and estrogen; Oxidoreductase
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle
Molecular Function: 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; testosterone 17-beta-dehydrogenase (NADP+) activity
Biological Process: androgen biosynthetic process; male genitalia development
Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
Research Articles on HSD17B3
1. Mutation G133R in HSD17B3 results in almost complete loss of enzyme activity since it interferes with binding of cofactor NADPH.
Precautions
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Disclaimer
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