Product Name
SMARCA2, siRNA
Full Product Name
SMARCA2 siRNA (Mouse)
Product Synonym Names
BAF190B; BRM; SNF2A; SNF2L2; Probable global transcription activator SNF2L2; ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Product Gene Name
SMARCA2 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q6DIC0
Specificity
SMARCA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SMARCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SMARCA2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SMARCA2 sirna
siRNA to inhibit SMARCA2 expression using RNA interference
Applications Tested/Suitable for SMARCA2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SMARCA2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_035546.2
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NCBI GenBank Nucleotide #
NM_011416.2
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UniProt Primary Accession #
Q6DIC0
[Other Products]
UniProt Related Accession #
Q6DIC0[Other Products]
Molecular Weight
180,253 Da
NCBI Official Full Name
probable global transcription activator SNF2L2 isoform 1
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
NCBI Official Symbol
Smarca2 [Similar Products]
NCBI Official Synonym Symbols
brm; Snf2l2; brahma; SNF2alpha; 2610209L14Rik
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NCBI Protein Information
probable global transcription activator SNF2L2
UniProt Protein Name
Probable global transcription activator SNF2L2
UniProt Synonym Protein Names
ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Protein Family
Probable global transcription activator
UniProt Gene Name
Smarca2 [Similar Products]
UniProt Synonym Gene Names
BAF190B [Similar Products]
UniProt Entry Name
SMCA2_MOUSE
UniProt Comments for SMARCA2
SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.
Protein type: Helicase; EC 3.6.1.-; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 3.6.4.-
Cellular Component: nucleoplasm; SWI/SNF complex; intermediate filament cytoskeleton; intracellular membrane-bound organelle; nuclear chromatin; nucleus
Molecular Function: protein binding; hydrolase activity, acting on acid anhydrides; DNA binding; histone binding; hydrolase activity; ATPase activity; nucleotide binding; chromatin binding; helicase activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
Biological Process: chromatin remodeling; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; nervous system development; nucleosome assembly; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent
Research Articles on SMARCA2
1. Brm protects from UV-induced hyperplastic growth in both cutaneous and corneal keratinocytes
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
