Product Name
TRAPPC9, siRNA
Full Product Name
TRAPPC9 siRNA (Mouse)
Product Synonym Names
KIAA1882; NIBP; Trafficking protein particle complex subunit 9; NIK- and IKBKB-binding protein
Product Gene Name
TRAPPC9 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q3U0M1
Specificity
TRAPPC9 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TRAPPC9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of TRAPPC9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
TRAPPC9 sirna
siRNA to inhibit TRAPPC9 expression using RNA interference
Applications Tested/Suitable for TRAPPC9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for TRAPPC9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001158113.1
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NCBI GenBank Nucleotide #
NM_001164641.1
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UniProt Primary Accession #
Q3U0M1
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UniProt Secondary Accession #
Q3UU81; Q69Z79; Q6NS50; Q8CD01; Q8CFV8; Q9D8R6[Other Products]
UniProt Related Accession #
Q3U0M1[Other Products]
Molecular Weight
56,240 Da
NCBI Official Full Name
trafficking protein particle complex subunit 9 isoform 3
NCBI Official Synonym Full Names
trafficking protein particle complex 9
NCBI Official Symbol
Trappc9 [Similar Products]
NCBI Official Synonym Symbols
Ibp; Nibp; TRS130; mKIAA1882; 1810044A24Rik; 2900005P22Rik; 4632408O18Rik
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NCBI Protein Information
trafficking protein particle complex subunit 9
UniProt Protein Name
Trafficking protein particle complex subunit 9
UniProt Synonym Protein Names
NIK- and IKBKB-binding protein
Protein Family
Trafficking protein particle complex
UniProt Gene Name
Trappc9 [Similar Products]
UniProt Synonym Gene Names
Kiaa1882; Nibp [Similar Products]
UniProt Entry Name
TPPC9_MOUSE
UniProt Comments for TRAPPC9
NIBP: Functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. May function in neuronal cells differentiation. May play a role in vesicular transport from endoplasmic reticulum to Golgi. Defects in TRAPPC9 are the cause of mental retardation autosomal recessive type 13 (MRT13). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Brain magnetic resonance imaging of MRT13 patients indicates the presence of mild cerebral white matter hypoplasia. Microcephaly is present in some but not all affected individuals. Belongs to the NIBP family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Activator
Cellular Component: Golgi apparatus; endoplasmic reticulum; cytoplasm
Biological Process: cell differentiation
Research Articles on TRAPPC9
1. NIBP is extensively expressed in the enteric nervous system with relatively high level in a subpopulation of enteric neurons.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
