Product Name
SGLT-1, Polyclonal Antibody
Full Product Name
SGLT-1 Antibody
Product Synonym Names
SGLT 1; SLC5A1; Sodium glucose cotransporter 1; D22S675; NAGT
Product Gene Name
anti-SGLT-1 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P13866
Purity/Purification
Affinity purified
Form/Format
100 ug (0.5 mg/ml) protein A affinity purified rabbit polyclonal SGLT-1 antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Appearance: Colorless liquid
Concentration
0.5 mg/ml (lot specific)
Immunogen
Synthetic peptide surrounding amino acid 594 of human SGLT-1
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Other Notes
Small volumes of anti-SGLT-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SGLT-1 antibody
Background: Glucose transporters are integral membrane proteins that mediate the transport of glucose and structurally related substances across cellular membranes. Two families of glucose transporter have been identified: the facilitated diffusion glucose transporter family (GLUT family), also known as 'uniporters,' and the sodium dependent glucose transporter family (SGLT family), also known as 'cotransporters' or 'symporters'. The SGLT1 gene encodes a protein that is involved in the active transport of glucose and galactose into eukaryotic and some prokaryotic cells.
Applications Tested/Suitable for anti-SGLT-1 antibody
Western Blot (WB)
Application Notes for anti-SGLT-1 antibody
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody detects 73 kDa SGLT-1 from human samples and in a lesser extent from mouse and rat samples. A ~50 kDa cleavage fragment can also be detected in Jurkat cell lysate.
Testing Data of anti-SGLT-1 antibody
NCBI/Uniprot data below describe general gene information for SGLT-1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000334.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000343.3
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UniProt Primary Accession #
P13866
[Other Products]
UniProt Secondary Accession #
B2R7E2; B7Z4Q9; B7ZA69[Other Products]
UniProt Related Accession #
P13866[Other Products]
Molecular Weight
60,097 Da
NCBI Official Full Name
sodium/glucose cotransporter 1 isoform 1
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/glucose cotransporter), member 1
NCBI Official Symbol
SLC5A1 [Similar Products]
NCBI Official Synonym Symbols
NAGT; SGLT1; D22S675
[Similar Products]
NCBI Protein Information
sodium/glucose cotransporter 1
UniProt Protein Name
Sodium/glucose cotransporter 1
UniProt Synonym Protein Names
High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1
UniProt Gene Name
SLC5A1 [Similar Products]
UniProt Synonym Gene Names
NAGT; SGLT1; Na(+)/glucose cotransporter 1 [Similar Products]
UniProt Entry Name
SC5A1_HUMAN
NCBI Summary for SGLT-1
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
UniProt Comments for SGLT-1
SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q12.3
Cellular Component: integral to plasma membrane; brush border membrane; apical plasma membrane; plasma membrane; intercellular junction
Molecular Function: protein binding; glucose:sodium symporter activity
Biological Process: hexose transport; sodium ion transport; carbohydrate metabolic process; pathogenesis; glucose transport; transmembrane transport; metanephros development; intestinal absorption
Disease: Glucose/galactose Malabsorption
Research Articles on SGLT-1
1. SGLT1 or GLUT2 interact with the cytoskeleton in the intestinal epithelium during hexose absorption.
Precautions
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Disclaimer
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