Product Name
14-3-3 protein zeta (14-3-3zeta), Recombinant Protein
Popular Item
Full Product Name
Recombinant Drosophila melanogaster 14-3-3 protein zeta (14-3-3zeta)
Product Gene Name
14-3-3zeta recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-248aa, full length protein
Sequence
MSTVDKEELV QKAKLAEQSE RYDDMAQAMK SVTETGVELS NEERNLLSVA YKNVVGARRS SWRVISSIEQ KTEASARKQQ LAREYRERVE KELREICYEV LGLLDKYLIP KASNPESKVF YLKMKGDYYR YLAEVATGDA RNTVVDDSQT AYQDAFDISK GKMQPTHPIR LGLALNFSVF YYEILNSPDK ACQLAKQAFD DAIAELDTLN EDSYKDSTLI MQLLRDNLTL WTSDTQGDEA EPQEGGDN
3D Structure
ModBase 3D Structure for P29310
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
>85% (SDS-PAGE) (lot specific)
Form/Format
Liquid containing glycerol
Tag Information
This protein contains an N-terminal tag and may also contain a C-terminal tag. Tag types are determined by various factors including tag-protein stability, please inquire for tag information.
Sterility
Sterile filter available upon request.
Endotoxin
Low endotoxin available upon request.
Species
Drosophila melanogaster (Fruit fly)
Storage Buffer
Tris-based buffer, 50% glycerol
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of 14-3-3zeta recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
NCBI/Uniprot data below describe general gene information for 14-3-3zeta. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001014515.1
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NCBI GenBank Nucleotide #
NM_001014515.2
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UniProt Primary Accession #
P29310
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UniProt Secondary Accession #
O01665; Q0E9D5; Q0E9D6; Q0E9D7; Q8IGB9; Q8MKV5; Q9V5G6; A4UZB2; A4UZB3; C1C593; C9QP27[Other Products]
UniProt Related Accession #
P29310[Other Products]
Molecular Weight
28,304 Da
NCBI Official Full Name
14-3-3zeta, isoform J
NCBI Official Symbol
14-3-3zeta [Similar Products]
NCBI Official Synonym Symbols
14-3-3; 14-3-3 zeta; 14-3-3-Leo; 14-3-3-zeta; 14-3-3leo; 14-3-3ZETA; 2G1; 4-3-3 zeta; 5.11; 549; BEST:GH05075; CG17870; D14-3-3; d14-3-3zeta; D14-3-3zeta; DmelCG17870; K; l(2)07103; l(2)46CFe; l(2)46Ee; leo; Leo; LEO; par-5; Par-5; PAR-5; PAR5
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NCBI Protein Information
CG17870 gene product from transcript CG17870-RE
UniProt Protein Name
14-3-3 protein zeta
UniProt Synonym Protein Names
14-3-3-like protein; Protein Leonardo
UniProt Gene Name
14-3-3zeta [Similar Products]
UniProt Synonym Gene Names
14-3-3; 14-3-3EZ; leo; THAP [Similar Products]
UniProt Comments for 14-3-3zeta
Required in Raf-dependent cell proliferation and photoreceptor differentiation during eye development (PubMed:9159395). Acts upstream of Raf and downstream of Ras, and is essential for viability (PubMed:9159395). Acts as a negative regulator of the slo calcium channel via its interaction with slo-binding protein slob (PubMed:10230800). Inhibits yki activity by restricting its nuclear localization (PubMed:19900439). Binds to and promotes the activity of phosphoinositide 3-kinase Pi3K68D which converts phosphatidylinositol to phosphatidylinositol-3-phosphate and promotes maturation of early endosomes (PubMed:27015288).
Research Articles on 14-3-3zeta
1. The Khc73 stalk/14-3-3/NudE pathway defines a physical connection that coordinates the activities of multiple motor proteins to precisely position the spindle.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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