3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, Polyclonal Antibody

HMG-CoA Reductase; Hydroxymethylglutaryl-CoA Reductase

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

The antibody is a rabbit polyclonal antibody raised against HMGCR. It has been selected for its ability to recognize HMGCR in immunohistochemical staining and western blotting.

Affinity Chromatography

Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.

200ug/ml (lot specific)

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Small volumes of anti-HMGCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot: Sample: Recombinant HMGCR, Rat.

DABstainingonIHC-P.Samples:RatTissue)

96,688 Da

3-hydroxy-3-methylglutaryl-CoA reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase

3-hydroxy-3-methylglutaryl-coenzyme A reductase

HMG-CoA reductase  [Similar Products]

enzyme involved in mevalonate synthesis [RGD, Feb 2006]

HMGCR: the rate-limiting enzyme for cholesterol synthesis. Regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by this reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis.

Protein type: EC 1.1.1.34; Endoplasmic reticulum; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Oxidoreductase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral component of membrane; intracellular membrane-bound organelle; peroxisomal membrane

Molecular Function: coenzyme binding; hydroxymethylglutaryl-CoA reductase (NADPH) activity; hydroxymethylglutaryl-CoA reductase activity; NADPH binding; protein homodimerization activity; protein phosphatase 2A binding

Biological Process: aging; cholesterol biosynthetic process; coenzyme A metabolic process; farnesyl diphosphate biosynthetic process, mevalonate pathway; isoprenoid biosynthetic process; myoblast differentiation; negative regulation of apoptosis; negative regulation of blood vessel diameter; negative regulation of MAP kinase activity; negative regulation of striated muscle cell apoptotic process; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of skeletal muscle tissue development; positive regulation of smooth muscle cell proliferation; positive regulation of stress-activated MAPK cascade; protein tetramerization; response to ethanol; response to nutrient; ubiquinone metabolic process; visual learning

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