Butyrate Response Factor 1/2, Polyclonal Antibody

Anti -Butyrate Response Factor 1/2 (BRF 1/2)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 14; NC_000014.8 (69254375..69259785, complement). Location: 14q22-q24

Polyclonal

IgG

Rabbit

Recognizes endogenous levels of total human BRF1 and BRF2 proteins. Species Crossreactivity: mouse, rat and monkey.

Affinity Purified
Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-ZFP36L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 family of CCCH zinc-finger proteins. This family of proteins, which also includes tristetraprolin (TTP), bind to AU-rich elements (ARE) found in the 3'-untranslated regions of mRNAs and promote de-adenylation and rapid degradation by the exosome. These proteins play a critical role in cell growth control by regulating the mRNA turnover of multiple cytokines, growth factors and cell cycle regulators, including GM-CSF, TNF alpha, IL-2, IL-3 and IL-6. Deregulated ARE-mRNA stability can contribute to both inflammation and oncogenic transformation. Insulin-induced stabilization of ARE-containing transcripts is mediated by Akt/PKB phosphorylation of BRF1 at Ser92, which results in binding by 14-3-3 protein and inactivation of BRF1.

Antibodies; Abs to Transcription Factors

ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry.
Dilution: Western Blot: 1:1000
Immunofluorescence (IF-IC): 1:25
Flow Cytometry: 1:100

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Zinc finger protein 36, C3H1 type-like 1

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TISB_HUMAN

This gene is a member of the TIS11 family of early response genes. Family members are induced by various agonists such as the phorbol ester TPA and the polypeptide mitogen EGF. The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The encoded protein contains a distinguishing putative zinc finger domain with a repeating cys-his motif. This putative nuclear transcription factor most likely functions in regulating the response to growth factors. [provided by RefSeq]

BRF1: a zinc finger protein that plays an important role in the assembly of the RNA polymerase III initiation factor TFIIIB. Regulates mRNA levels by targeting transcripts containing AREs (AU-rich elements) into the decay pathway. Phosphorylation by Akt apparently generates 14-3-3 s and inhibits BRF1 from promoting mRNA activity.Contains 2 C3H1-type zinc fingers.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 14q22-q24

Cellular Component: nucleus; cytosol

Molecular Function: mRNA binding; protein binding; DNA binding; metal ion binding; transcription factor activity

Biological Process: neural tube development; regulation of translation; cell proliferation; regulation of transcription, DNA-dependent; apoptosis; multicellular organism growth; heart development; T cell differentiation in the thymus; gene expression; regulation of mRNA stability; vasculogenesis; mRNA catabolic process, deadenylation-dependent decay

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