Slc5a1, Polyclonal Antibody

Slc5a1; solute carrier family 5 (sodium/glucose cotransporter), member 1; MGC93553; Sglt1; Na(+)/glucose cotransporter 1; Solute carrier family 5, member alpha 1 (Na+/glucose cotransporter); high affinity sodium-glucose cotransporter; sodium/glucose cotra; high affinity sodium-glucose cotransporter antibody; MGC93553 antibody; Na(+)/glucose cotransporter 1 antibody; Sglt1 antibody; sodium/glucose cotransporter 1 antibody; solute carrier family 5 (sodium/glucose cotransporter); member 1 antibody; solute carrier family 5; member 1 antibody; Solute carrier family 5; member alpha 1 (Na+/glucose cotransporter) antibody; Slc5a1 antibody; Slc5a1 (rat)

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul (lot specific)

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Manufactured in an ISO 9001:2008 Certified Laboratory.

Small volumes of anti-Slc5a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: Cryosections of paraformaldehyde fixed Rat Kidney shows strong staining of the brush-border in proximal straight tubules (S3 segments). Cryosections of paraformaldehyde fixed Rat Small Inteestines shows staining of the brush-border of absorptive cells. Recommended concentration, 20-30ug/ml. Data provided by Ivana Vrhovac, B. Sc. and Ivan Sabolic, M.D., Ph.D., IMROH, Zagreb, Croatia.
Western Blot: Approx 75-80kDa band observed in isolated Brush Border Membranes (BBM) of female Rat Kidney and approx 80kDa in BBM of female Rat Small Intestine (calculated MW of 73.1kDa according to NP_037165.1). Recommended concentration: 1-3ug/ml. Data provided by Ivana Vrhovac, B. Sc. And Ivan Sabolic, M. D. , Ph. D. , IMROH, Zagreb, Croatia.

(2.5ug/ml) Western blot staining of Brush-border membranes from Rat Kidney. Primary incubation was 1 hour. Detected by chemiluminescence.

(2.5ug/ml) Western blot staining of Brush-border membranes from Rat Small Intestines. Primary incubation was 1 hour. Detected by chemiluminescence.

73,066 Da

solute carrier family 5 member 1

sodium/glucose cotransporter 1

Sodium/glucose cotransporter 1

Sglt1; Na(+)/glucose cotransporter 1  [Similar Products]

SC5A1_RAT

catalyzes sodium dependent glucose transport; plays a role in sugar absorption in the small intestine and reabsorption of sugars in the kidney [RGD, Feb 2006]

SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Cellular Component: apical plasma membrane; brush border; brush border membrane; integral to plasma membrane; intercellular junction

Molecular Function: glucose transmembrane transporter activity; glucose:sodium symporter activity

Biological Process: D-glucose absorption; glucose transport; intestinal absorption; metanephros development; sodium ion transport

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