Full Product Name
C-myc HRP Conjugated Antibody
Product Gene Name
anti-C-myc antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01106
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the residues of c-myc.
Purity/Purification
Affinity Purified
Cellular Localization
Cell Nuclear
Positive Control
Recombinant protein
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-C-myc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-C-myc antibody
Myc gene encodes for a transcription factor that is believed to regulate expression of 15% of all genes through binding on Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. This c-Myc antibody detects endogenous levels of total c-Myc protein and is also for detection of Myc-tagged fusion protein.
Applications Tested/Suitable for anti-C-myc antibody
Western Blot (WB)
Application Notes for anti-C-myc antibody
WB: 1-1,000-1:2,000
Testing Data of anti-C-myc antibody
Western blot analysis on myc-tagged recombinant protein using c-myc antibody HRP conjugated.
NCBI/Uniprot data below describe general gene information for C-myc. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002458.2
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NCBI GenBank Nucleotide #
NM_002467.5
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UniProt Primary Accession #
P01106
[Other Products]
UniProt Secondary Accession #
P01107; Q14026; A8WFE7[Other Products]
UniProt Related Accession #
P01106[Other Products]
NCBI Official Full Name
myc proto-oncogene protein isoform 1
NCBI Official Synonym Full Names
MYC proto-oncogene, bHLH transcription factor
NCBI Official Symbol
MYC [Similar Products]
NCBI Official Synonym Symbols
MRTL; MYCC; c-Myc; bHLHe39
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NCBI Protein Information
myc proto-oncogene protein
UniProt Protein Name
Myc proto-oncogene protein
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Protein Family
C-Myc-binding protein
UniProt Gene Name
MYC [Similar Products]
UniProt Synonym Gene Names
BHLHE39; bHLHe39 [Similar Products]
NCBI Summary for C-myc
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini. [provided by RefSeq, Aug 2017]
UniProt Comments for C-myc
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000).
Product References and Citations for anti-C-myc antibody
1. "A quantitative atlas of mitotic phosphorylation."Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). 2. "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62."Gupta S., Seth A., Davis R.J. Proc. Natl. Acad. Sci. U.S.A. 90:3216-3220(1993).
Research Articles on C-myc
1. mdig directly interacts with c-myc and JAK1 in multiple myeloma (MM) cell lines, which contributes to hyperactivation of the IL-6-JAK-STAT3 signaling important for the pathogenesis of MM.
Precautions
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Disclaimer
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