Full Product Name
c-Myc Polyclonal Antibody
Product Synonym Names
MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Product Gene Name
anti-c-Myc antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P01106
Species Reactivity
Human, Mouse, Rat
Specificity
c-Myc Polyclonal Antibody detects endogenous levels of c-Myc protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Immunogen Description
Synthesized peptide derived from human c-Myc around the non-phosphorylation site of S62.
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-c-Myc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-c-Myc antibody
Total protein Ab
Applications Tested/Suitable for anti-c-Myc antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes for anti-c-Myc antibody
Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:40000
Not yet tested in other applications.
Western Blot (WB) of anti-c-Myc antibody
Western Blot analysis of HeLa cells using c-Myc Polyclonal Antibody
NCBI/Uniprot data below describe general gene information for c-Myc. It may not necessarily be applicable to this product.
NCBI Accession #
NP_002458.2
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NCBI GenBank Nucleotide #
NM_002467.4
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UniProt Primary Accession #
P01106
[Other Products]
UniProt Secondary Accession #
P01107; Q14026; A8WFE7[Other Products]
UniProt Related Accession #
P01106[Other Products]
Molecular Weight
50,565 Da
NCBI Official Full Name
myc proto-oncogene protein
NCBI Official Synonym Full Names
v-myc avian myelocytomatosis viral oncogene homolog
NCBI Official Symbol
MYC [Similar Products]
NCBI Official Synonym Symbols
MRTL; MYCC; c-Myc; bHLHe39
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NCBI Protein Information
myc proto-oncogene protein
UniProt Protein Name
Myc proto-oncogene protein
UniProt Synonym Protein Names
Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Protein Family
C-Myc-binding protein
UniProt Gene Name
MYC [Similar Products]
UniProt Synonym Gene Names
BHLHE39; bHLHe39 [Similar Products]
UniProt Entry Name
MYC_HUMAN
NCBI Summary for c-Myc
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for c-Myc
Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.
Protein type: Oncoprotein; DNA-binding; Transcription factor; Nucleolus
Chromosomal Location of Human Ortholog: 8q24.21
Cellular Component: nucleoplasm; protein complex; nucleolus; cytosol; nucleus
Molecular Function: protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor activity; transcription factor binding
Biological Process: oxygen transport; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; Wnt receptor signaling pathway through beta-catenin; negative regulation of transcription from RNA polymerase II promoter; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; regulation of telomere maintenance; transcription, DNA-dependent; MAPKKK cascade; negative regulation of cell division; negative regulation of monocyte differentiation; negative regulation of stress-activated MAPK cascade; chromatin remodeling; negative regulation of fibroblast proliferation; ureteric bud branching; regulation of gene expression; energy reserve metabolic process; positive regulation of transcription from RNA polymerase II promoter; gene expression; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis
Disease: Burkitt Lymphoma
Research Articles on c-Myc
1. B-cell tumors harbored oncogenic c-myc amplification generated by breakage-fusion-bridge (BFB) from dicentric chromosomes formed through fusion of IgH V(D)J recombination-associated DSBs on chromosome 12 to sequences downstream of c-myc on chromosome 15.
Precautions
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