Product Name
SLC5A1, siRNA
Full Product Name
SLC5A1 siRNA (Rat)
Product Synonym Names
SGLT1; Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1
Product Gene Name
SLC5A1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P53790
Specificity
SLC5A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC5A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC5A1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC5A1 sirna
siRNA to inhibit SLC5A1 expression using RNA interference
Applications Tested/Suitable for SLC5A1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC5A1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_037165.1
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NCBI GenBank Nucleotide #
NM_013033.2
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UniProt Primary Accession #
P53790
[Other Products]
UniProt Secondary Accession #
P97787[Other Products]
UniProt Related Accession #
P53790[Other Products]
Molecular Weight
73,066 Da
NCBI Official Full Name
sodium/glucose cotransporter 1
NCBI Official Synonym Full Names
solute carrier family 5 (sodium/glucose cotransporter), member 1
NCBI Official Symbol
Slc5a1 [Similar Products]
NCBI Official Synonym Symbols
SGLT1
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NCBI Protein Information
sodium/glucose cotransporter 1
UniProt Protein Name
Sodium/glucose cotransporter 1
UniProt Synonym Protein Names
High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1
Protein Family
Sodium/glucose cotransporter
UniProt Gene Name
Slc5a1 [Similar Products]
UniProt Synonym Gene Names
Sglt1; Na(+)/glucose cotransporter 1 [Similar Products]
UniProt Entry Name
SC5A1_RAT
NCBI Summary for SLC5A1
catalyzes sodium dependent glucose transport; plays a role in sugar absorption in the small intestine and reabsorption of sugars in the kidney [RGD, Feb 2006]
UniProt Comments for SLC5A1
SLC5A1: Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. Expressed mainly in intestine and kidney. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral
Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; intercellular junction; brush border
Molecular Function: glucose:sodium symporter activity; glucose transmembrane transporter activity
Biological Process: D-glucose absorption; sodium ion transport; glucose transport; metanephros development; transmembrane transport; intestinal absorption
Research Articles on SLC5A1
1. Chronic and direct silencing of basolateral Na-K-ATPase uniquely regulates brush border membrane Na absorptive pathways in intestinal epithelial cells. Specifically, SGLT1 is stimulated secondary to enhanced affinity of the cotransporter.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
